Microbiological Analysis

 

TESTDescriptionMethod
Microscopic identification
Yeast and bacteria identificationSpecies identificationPCR Scorpion™
Brettanomyces cultureChecks for viable yeast, specifically BrettanomycesPlating on WLC media
Bacteria cultureChecks for viable bacteriaPlating on MR media
Yeast cultureChecks for viable yeastPlating on WL media
Yeast countMonitor fermentationsHaemocytometry
Yeast viabilityMonitor fermentationsStaining
Sterility3-day sterility check for bottled/packaged samplesFilter plating
5-day sterility check for bottled/packaged samplesFilter plating
Alcohol-tolerance of yeastCheck growth in 10% ethanol medium
Additional analysis for export requirements
Total plate countStandard TTC
Total coliforms and E.coliEndo
StaphylococcusChapman
Yeast and MouldWLN
Acetic acid bacteriaMR
Lactic acid bacteriaMR
PANEL: Slow/stuck fermentations Microscopic ID; Yeast count; Yeast viability; Glucose; Fructose; Malic acid
PANEL: Stuck MLF Microscopic ID; Bacteria culture; Malic acid; Alcohol; pH; Total SO2
PANEL: Brettanomyces management  4-EP, 4-EG; Brettanomyces culture
PANEL: Filtration decisionsMicroscopic ID; Bacteria culture; Brettanomyces culture; Glucose; Fructose; Malic acid; VA; Turbidity; pH